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It is used as a purifying process for differential centrifugation.
The organelles are then generally separated using differential centrifugation.
Microsomes can be concentrated and separated from other cellular debris by differential centrifugation.
Differential centrifugation Media for cell separation by density:
Before differential centrifugation can be carried out to separate different portions of a cell from one another, the tissue sample must first be homogenised.
Invariably achieved by Differential centrifugation - the sequential increase in gravitational force results in the sequential separation of organelles according to their density.
Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells.
Variations of this include Isopycnic centrifugation, Differential centrifugation, and Sucrose gradient centrifugation.
PBMCs were separated from blood on Ficoll-Paque by differential centrifugation and were suspended in 24-well tissue culture plates culture medium.
Differential centrifugation of the homogenate showed that the midasin remained in the supernatant fraction upon centrifugation at 2,000 x g for 2 min, but mostly passed into the pellet fraction upon centrifugation at 18,000 x g for 20 min.
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